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Reducing Volatile Organic Compounds in Silages

06 March 2012

Biomin

Volatile Organic Compounds (VOC) can have short and long-term adverse health effects but can also impact feed intake. Biomin looks at how VOCs can be reduced in silages using inoculants.

What are VOC´s?

Volatile Organic Compounds (VOC´s) are emitted as gas from certain solids or liquids. VOCs include a variety of chemicals, some of which may have short- and long-term adverse health effects (1), which evaporate relatively easily. Regulations in different countries define VOC´s as substances with a boiling point of up to 250°C. Of course, their concentrations in the air are much higher indoors than outdoors.

Many studies about the dangerous effects on human health and the environment have been carried out in the past. VOC´s surround us. Normally they do not act acutely but long term. They are considered to be air pollutants and often symptoms like allergies, respiratory diseases,irritation, immune depression, nauseas, and many others have been reported. An example is the high level of ozone in Central California,USA (2) which is caused by the reaction between VOC´s and nitrogen oxides in the presence of sunlight, which leads to new ozone standards (3).

Research in animal production has been focusing on VOC emissions from agricultural sources (4) and its impact on, for example, feed intake (5). The impact on animal health has been less investigated due to the obvious difficulties regarding an adequate trial design. However, more research is needed in this area as high yield animals are kept indoors most of the time and air quality could be a limiting factor to achieving optimal performance.

Production of VOC´s in the silage

VOC´s are mainly produced by plants, as well as animals and microorganisms. In order to reduce the production of VOC´s it is important to monitor their concentrations and correlate them with other physical factors such as temperature, sunlight, decomposition of organic matter, etc. The reduction of ozone contamination, for example, was more effective in urban areas than in rural areas, because in urban areas the VOC´s are a limiting factor, whereas in rural areas it is the opposite (4).

The scientific community has been paying more and more attention to silage, as a source of VOC´s, in the last year. Silages are considered to produce more than 700 substances which are potentially dangerous for the formation of ozone (4). One of the major problems within this topic is the countless possible combinations of factors which can contribute to an increased production of VOC´s. As explained before, the term ‘VOC´s’ is a common term for thousands of substances which could represent a potential damage and this fact leaves us with very costly quantitative analysis.

There are several sources of VOC´s in dairy production: crops, feed production, preserved feed through fermentation, in silos, due to decomposition of organic material, during digestion, oxidation lagoons, manure, etc.

Several VOC´s have been identified in silages (see list below).

List of the most well-known VOC´s in silages:

  • Isoprene (C5H8)
  • Monoterpenes(C10H16)
  • Oxygenated VOC
  • Sesquiterpenes(C15H24)
  • Alcohol (ethanol, methanol, propanol, butanol)
  • Esters (hexyl-, butyl-, propyl-, methyl- and ethyl- acetate; propyl-, ethyl- propionate; butyl-, propyl-, ethyl- butyrate; propyl-, ethyl- hexanoate)
  • Aldehydes
  • Ketones
  • Carbonyl compound emissions

However, the identification of those VOC’s of major significance in silage production, as indicators of pollution from a practical point of view, is a must. Researchers mainly discovered emissions of alcohols [mainly ethanol, (5, 6)], volatile fatty acids, esters and aldehydes. Even when ethanol has not had a major effect as a VOC, the amounts produced in unstable silages can be relatively high (8 – 10% in DM in silages with aerobic instability). Other dangerous substances were also found such as alkenes, alkynes, etc. and esters as the majority of VOC´s in silages (7).

Ethanol emission rate is affected by several factors in the silage. Ethanol is the main product of yeast metabolism and represents a fermentation process in which approx. 40% of the energy is lost (8). Good agricultural practices (right harvest time, good compaction, proper silo sealing using sheets with low permeability, sufficient weights on the silo covering, adequate silo design, adequate advance in the silo in the feed out phase (which decreases the exposed silage layer), certain levels of acetic acid, clean-cut surface) should diminish the presence of ethanol in the silage. High contribution to total ozone formation from different types of silages (corn, alfalfa, cereal) has been associated with the alcohol content (9), especially ethanol, which is considered a dominant silage VOC (10, 11).

Focus on reduction of the VOC ethanol from silages

The reduction of ethanol from silages is a major issue, not only for the reduction of an important source of VOC´s but also to increase the profitability of a farm. As discussed before, the secondary fermentation by yeasts and formation of ethanol causes enormous losses due to aerobic instability in the silage.

An estimation of ethanol production from silages in Western Europe, based on the silage production [500 million tons (12)], 30% of DM and an ethanol content of 1% (8) (in unstable silages this quantity is higher) amounts to 1.5 million tons of ethanol per year. Therefore, not due to its qualitative importance as a VOC, which is relatively low, but because of the huge quantities of ethanol produced, this should be focused on more in the silage production and use process.

Ethanol emission depends on exposure time, ambient temperature and air velocity (6). Another important factor is the porosity of the silages, whose density and structure was disrupted, resulting in a lower density and higher porosity, as occurred in the feed out phase (13).

As discussed before, the amount of VOC´s is higher indoors than outdoors. This would mean that when unstable silage is brought to the stable it would be more dangerous for animal health because of the duration of exposure at increased rates. Even when the evidence is not fully corroborated, and mostly in animal bioassays, it is considered that many organic compounds are associated with or suspected to cause cancer in animals (14). However, many VOC´s are identified as carcinogenic substances in humans (15), therefore there is also a risk to animals.

The interaction between different VOC´s can increase the risk for animals which have a longer productive cycle, for instance, dairy cows. There is evidence regarding high correlations between esters and ethanol found in corn silages [r2 = 0.52 and 0.78 for ethyl acetate and ethyl lactate x ethanol respectively (16)]. It is perfectly viable that other VOC´s can be also correlated or come in contact with those from silages in the stable.

The contribution of silage inoculants to the reduction of VOC production

Meanwhile, the use of silage inoculants is a common practice in the silage production. Homolactic lactic acid bacteria (LAB) are used as fermentation enhancers, whereas heterolactic LAB are mainly included for preventing aerobic instability in the feed out phase. Alcohol (ethanol) is produced primarily by yeast in that phase. It is already very well-known and documented that acetic acid from heterolactic LAB inhibits the growth of yeast under aerobic conditions (Table 1).

Table 1: Effect of Acetic Acid on Different Yeasts

Yeast Author Year Statement
Saccharomyces rouxii and Torulopsis versatilis Noda et al. 1982 Acetic acid has an increased toxic effect on yeasts in brine fermentation of soy sauce from pH 5.5 to 3.5
Candida krusei and Pichia subpelliculosa Danner et al. 2003 Acetic acid has the greatest inhibitory effect on yeast growth. 20 g liter?1 of acetic acid in the test mixture was enough to completely inhibit the growth of the selected yeasts at pH 4
Silage yeasts Driehuis and van Wikselaar Oude Elferink et al. 1996

1999
High levels of formic or acetic acid reduce survival of yeasts during storage (in silages)
Silage yeasts Driehuis et al. Oude Elferink et al. 1997

1999
Lactic acid is degraded anaerobically to acetic acid and 1,2-propanediol, which in turn causes a significant reduction in yeast numbers

In some studies the correlation coefficient between ethanol and acetic acid was negative, middle–low, however, highly significant [r2 = -0.23 (17)]. This relationship is shown in Diagram 1.

Relationship Between Acetic Acid and Ethanol in Corn Silages (18)

Even though acetic acid is also considered a VOC, its use shows many advantages which are described throughout scientific literature. Its use is therefore supported by a cost – benefit relationship.

Conclusions

As discussed above, interest in the research on VOC´s as air pollutants and their negative effects on human health is increasing.

Silages are an important source of VOC´s in animal production. Alcohols, especially ethanol, are major VOC´s in silages.

Ethanol emissions from silages on farms are increased by silage characteristics (type of silage, compacting, covering), ambient conditions (higher temperatures, increased air velocity, elongated time of silage exposure) and management practices.

The prevention of ethanol formation is based on the inhibition of yeasts in the feed out phase of the silos. For this purpose, the use of heterolactic LAB is a good ensiling practice to increase the acetic acid content of the silage.

Literature:

(1): EPA (United States Environmental Protection Agency. Available at: http://www.epa.gov/iaq/voc.html (accessed 07.10.2011)

(2): Huff Post Los Angeles. Available at: http://www.huffingtonpost.com/2011/08/23/california-central-valley-pollution_n_934485.html (accessed 11.10.2011)

(3): EPA (United States Environmental Protection Agency. Regulatory actions. Available at: http://www.epa.gov/glo/actions.html (accessed 07.10.2011)

(4): Mitloehner, F. M.; Malkina, I. L.; Kumar, A. and Green, P. G. (2009): Volatile organic compounds emitted from dairy silages and other feeds. Proceedings of the XVth International Silage Conference. July 27th – 29th 2009. Madison, Wisconsin, USA

(5): Krizsan, S. J.; Westad, F.; Adnøy, T.; Odden, E.; Aakre S. E. and Randby A. T. (2007): Effect of volatile compounds in grass silage on voluntary intake by growing cattle. Animal (2007), 1: Pages 283–292 Q. The Animal Consortium 2007

(6): Montes, F.; Hafner, S.; Rotz, A. C.; Mitloenher and F. M. (2010): Temperature and velocity effects on ethanol emissions from corn silage with the characteristics of an exposed silo face. Atmospheric environment (2010), doi: 10.1016/j.atmosenv.2010.02.037

(7): Mo, M.; Selmer-Olsen, I.; Randby, A.T.; Aakre, S.E. and Asmyhr, A. (2001): New fermentation products in grass silage and their effects on feed intake and milk taste. In: Proceedings of the 10th International Symposium on Forage Conservation, Brno, Czech Republic, 98–99

(8): McDonald, P., A.R. Henderson & S.J.E. Heron (1991). The biochemistry of silage. 2nd Edition. Chalcombe publications, Marlow Bucks, UK

(9): Howard, C. J.; Kumar, A.; Malkina, I. A.; Mitloehner, F. M.; Green, P. G.; Flocchini, R. and Kleeman, M. (2010): Reactive Organic Gas Emissions from Livestock Feed Contribute Significantly to Ozone Production in Central California. Env. Sci. & Technol. 44(7): 2309-2314

(10): Hafner, S. D.; Montes, F.; Rotz, C. A. and Mitloehner, F. (2010): Atmospheric Environment44, 4172-4180 (2010)

(11): Filipy, J., B. et al. (2006): Identification and quantification of volatile organic compounds from a dairy. Atmospheric Environment 40: 1480-1494

(12): Wilkinson, J. M. (2005): Silage. Chalcombe publications. ISBN 0 948617 50 0. p: 22

(13): Hafner, S. D.; Montes, F.; Rotz, C. A. and Mitloehner, F. (2010): Ethanol emission from loose corn silage and exposed silage particles. Atmospheric environment (2010), doi: 10.1016/j.atmosenv.2010.07.029

(14): Devine, J. (2011): The dangers of volatile organic compounds. EzineArticles.com. Expert Author. Available at:http://ezinearticles.com/?The-Dangers-of-Volatile-Organic-Compounds&id=3571066 (accessed 10.10.2011)

(15): EPA (United States Environmental Protection Agency. Regulatory actions. Available at: http://www.epa.gov/teach/chem_summ/VC_summary.pdf (accessed 01.10.2011)

(16): Weiß, K.; Kalzendorf, C.; Zittlau J. and Auerbach H. (2009): Novel results on the occurrence of volatile compounds in maize silages. Proceedings of the XVth International Silage Conference. July 27th – 29th 2009. Madison, Wisconsin, USA

(17): Acosta Aragón, Y.; Boeck, G.; Klimitsch, F.; Schatzmayr, G. and Pasteiner, S. (2008): Aerobic stability and silage quality parameters. Journal of Animal Sciences. Vol. 86, E-supplement. 2/ Journal of Animal Sciences, Vol. 91, E-supplement 1

(18): Acosta Aragón, Y.; Boeck, G.; Klimitsch, F.; Schatzmayr, G. and Pasteiner, S. (2011) (data not published)

March 2012

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